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anti brca1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti brca1
    Anti Brca1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+brca1/pm41900138-252-17-25
    Average 86 stars, based on 1 article reviews
    anti brca1 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Epacadostat and Olaparib Synergistically Inhibit the Growth of BRCA-Proficient Triple-Negative Breast Cancer by Suppressing the Expression of BRCA1 and RAD51.
    Article Snippet: Total proteins were extracted from MDA-MB-468 cells and MDA-MB-468 tumor xenografts using RIPA cell lysis buffer (Beyotime, Shanghai, China) supplemented with a protease and phosphatase inhibitor cocktail. .. The membranes were blocked with 5% (w/v) non-fat dry milk and then incubated with primary antibodies, including anti-BRCA1 (1:1000), anti-RAD51 (1:1000), and anti-β-Tubulin (1:5000) from Cell Signaling Technology (Beverly, MA). .. After centrifugation, the supernatant was carefully removed, and protein concentration was determined using the BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA).

    Article Title: Epacadostat and Olaparib Synergistically Inhibit the Growth of BRCA-Proficient Triple-Negative Breast Cancer by Suppressing the Expression of BRCA1 and RAD51
    Article Snippet: Briefly, proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then electrotransferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, MA, USA). .. The membranes were blocked with 5% ( w / v ) non-fat dry milk and then incubated with primary antibodies, including anti-BRCA1 (1:1000), anti-RAD51 (1:1000), and anti-β-Tubulin (1:5000) from Cell Signaling Technology (Beverly, MA). ..

    Proximity Ligation Assay:

    Article Title: ssDNA-PLA, a proximity ligation assay to interrogate DNA damage response proteins involved in homologous recombination
    Article Snippet: .. Anti-RPA2 phospho S33 (Abcam, ab211877, PLA 1:1000), Anti-RAD51 (Abcam, ab133534, PLA 1:1000), Anti-BRCA1 (Cell Signaling Technology, IB; 1:1000), Anti-BLM (Invitrogen, PA5-77880; PLA 1:200), Anti-BrdU (Roche, 11170376001, PLA 1:100, primarily used in this study), Anti-BrdU (BD Biosciences, Clone B44, Cat 347580, PLA 1:100, used in ), and anti-tubulin (Proteintech, 66240-1-Ig, IB 1:10000) were purchased. .. BrdU, etoposide (ETO), camptothecin (CPT), and hydroxyurea (HU) were purchased from Sigma-Aldrich.

    Article Title: ssDNA-PLA, a proximity ligation assay to interrogate DNA damage response proteins involved in homologous recombination
    Article Snippet: .. Anti-RPA2 phospho S33 (Abcam, ab211877, PLA 1:1000), Anti-RAD51 (Abcam, ab133534, PLA 1:1000), Anti-BRCA1 (Cell Signaling Technology, IB; 1:1000), Anti-BLM (Invitrogen, PA577880; PLA 1:200), Anti-BrdU (Roche, 11170376001, PLA 1:100, primarily used in this study), Anti-BrdU (BD Biosciences, Clone B44, Cat 347580, PLA 1:100, used in Fig. 4C) and antitubulin (Proteintech, 66240-1-Ig, IB 1:10000) were purchased. .. BrdU, Etoposide (ETO), camptothecin (CPT), and hydroxyurea (HU) were purchased from Sigma-Aldrich.

    other:

    Article Title: Pimitespib, an HSP90 Inhibitor, Enhances the Efficacy of PARP Inhibitors in PARP Inhibitor-Insensitive Breast Cancer Cells.
    Article Snippet: Anti- BRCA1 (#9010), anti- BRCA2 (#10741), anti- Rad51 (#8875), anti- p- histone H2A.X (#2577), anti- p- AKT (#4060), anti- AKT (#4691), anticleaved PARP (#5625), and antiGAPDH (#5174) antibodies were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA).



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